| 01:45 | Biggleswade FC – Three Bridges | 1–3 |
| 02:00 | Aveley – Ware | 3–1 |
| 21:00 | Hanwell Town – Enfield Town | 2–4 |
| 21:00 | Poole Town – Basingstoke | 1–0 |
| 21:00 | Newmarket Town – Leighton Town | 2–1 |
| 21:00 | Waltham Abbey – Afc Croydon Athletic | 7–1 |
| 21:00 | Real Bedford – Wingate & Finchley | 0–3 |
| 21:00 | Takeley – Hitchin Town | 2–0 |
| 21:00 | Havant & waterloovile – Taunton Town | 2–3 |
| 21:00 | Eastbourne Borough – Welwyn Garden City | 4–2 |
| 21:00 | Maldon & Tiptree – Egham Town | 0–2 |
| 21:00 | Moneyfields – Wimborne Town | 1–3 |
| 21:00 | Berkhamsted – Chatham Town | 3–1 |
| 21:00 | Plymouth Parkway – Bath City | 0–3 |
| 21:00 | Leiston – Dartford | 0–3 |
| 21:00 | Hastings Utd – Dulwich Hamlet | 2–1 |
| 21:00 | Canvey Island – Needham Market | 0–3 |
| 21:00 | St Albans City – Bury | 4–1 |
| 21:00 | Halesowen Town – Wellingborough Town | 2–0 |
| 21:00 | Alfreton – Shifnal Town | 2–1 |
| 21:00 | Hanworth Villa – Bishop's Stortford | 3–2 |
| 21:00 | Gloucester – Stafford Rangers | 4–0 |
| 21:00 | Burgess Hill Town – Winslow United | 6–2 |
| 21:00 | Whyteleafe – Whitehawk | 1–4 |
| 21:00 | Racing Club Warwick – Bromsgrove Sporting | 0–1 |
| 21:00 | Uxbridge – Lewes | 2–1 |
| 21:00 | Peterborough Sports – Banbury United | 1–3 |
| 21:00 | Quorn – Leamington | 4–2 |
| 21:00 | Ilkeston Town – Anstey Nomads | 1–3 |
| 21:00 | Stanway Rovers – Faversham Town | 0–2 |
| 21:00 | Tiverton Town – Gosport Borough | 1–3 |
| 21:00 | Thame United – Chichester City | 1–2 |
| 21:00 | Evesham Utd – Stratford Town | 3–1 |
| 21:00 | Matlock Town – Kettering | 0–4 |
| 21:00 | Worcester – Leek Town | 0–2 |
| 21:00 | Stamford AFC – Loughborough University | 1–2 |
| 21:00 | Bedworth United – Redditch Utd | 0–1 |
| 21:00 | Rushall Olympic – Stourbridge | 1–3 |
| 21:00 | Alvechurch – Bourne Town | 1–2 |
| 21:00 | Curzon Ashton – Stalybridge | 3–1 |
| 21:00 | Bootle – Afc Emley | 5–0 |
| 21:00 | Bridlington Town – Whitby Town | 3–0 |
| 21:00 | Warrington Rylands – Stockton Town | 3–1 |
| 21:00 | Guiseley AFC – Bamber Bridge | 4–2 |
| 21:00 | Cleethorpes Town – Lancaster City | 5–1 |
| 21:00 | Dunston Uts – Avro | 0–2 |
| 21:00 | Guisborough Town – Redcar Athletic | 0–1 |
| 21:00 | Workington – Blyth Spartans | 4–2 |
| 21:00 | Hyde – FC United | 2–0 |
| 21:00 | St Albans City – Bury Town | 4–1 |